Chitosans are involved in the trophic interactions of protists

The data store contains the RAW data from the LC-MS measurement of the samples used for Figure 2 (A and C) from the planned publication " Chitosans are involved in the trophic interactions of protists"

In more detail Chitooligomer_Profile_Figure2c RAW Data is from the SynaptXS mass spectrometer (Waters) measurements of the chitinosanase (AaChio) (Kohlhoff et al. "Chitinosanase: A fungal chitosan hydrolyzing enzyme with a new and unusually specific cleavage pattern)  hydrolysis products of chitosan polymer standards FA 0- FA55 as well as the Orciraptor agilis and emptied algae cells. The samples were separated using a BEH HILIC column (Waters). The identification of the chitin and chitosan oligomers was performed using the software Skyline. 

The Data set "MS Data to calculate the FA_Figure 2a" are the RAW Data files from the SynaptXS mass spectrometer (Waters) of the FA analysis methods (Urs et al. 2022 "Quantitative enzymatic-mass spectrometric analysis of the chitinous polymers in fungal cell walls"). The samples were separated using a BEH HILIC column (Waters). The intensity of the peaks corresponding to GlcNAc, GlcNAc-d3 and GlcNAc-d3-13C2 were used to quantify the amount of GlcNAc and GlcN in the two samples Orciraptor agilis and emptied algae cells. The calculation of these values as well as the FA of the samples are shown in the Excel document Evaluation_FA_Determination_Figure2a